Format

Send to

Choose Destination
Kidney Int. 2017 Aug;92(2):397-414. doi: 10.1016/j.kint.2017.02.001. Epub 2017 Apr 3.

Renal tubular NHE3 is required in the maintenance of water and sodium chloride homeostasis.

Author information

1
InterPrET Center, Department of Biomedicine, Aarhus University, Aarhus, Denmark.
2
InterPrET Center, Department of Biomedicine, Aarhus University, Aarhus, Denmark; VA San Diego Healthcare System, San Diego, California, USA.
3
VA San Diego Healthcare System, San Diego, California, USA.
4
Department of Medicine, University of Cincinnati, Cincinnati, Ohio, USA; Research Services, Veterans Affairs Medical Center, Cincinnati, Ohio, USA.
5
Department of Molecular Pharmacology and Physiology, University of South Florida, Tampa, Florida, USA.
6
Department of Molecular Pharmacology and Physiology, University of South Florida, Tampa, Florida, USA. Electronic address: trieg@health.usf.edu.

Abstract

The sodium/proton exchanger isoform 3 (NHE3) is expressed in the intestine and the kidney, where it facilitates sodium (re)absorption and proton secretion. The importance of NHE3 in the kidney for sodium chloride homeostasis, relative to the intestine, is unknown. Constitutive tubule-specific NHE3 knockout mice (NHE3loxloxCre) did not show significant differences compared to control mice in body weight, blood pH or bicarbonate and plasma sodium, potassium, or aldosterone levels. Fluid intake, urinary flow rate, urinary sodium/creatinine, and pH were significantly elevated in NHE3loxloxCre mice, while urine osmolality and GFR were significantly lower. Water deprivation revealed a small urinary concentrating defect in NHE3loxloxCre mice on a control diet, exaggerated on low sodium chloride. Ten days of low or high sodium chloride diet did not affect plasma sodium in control mice; however, NHE3loxloxCre mice were susceptible to low sodium chloride (about -4 mM) or high sodium chloride intake (about +2 mM) versus baseline, effects without differences in plasma aldosterone between groups. Blood pressure was significantly lower in NHE3loxloxCre mice and was sodium chloride sensitive. In control mice, the expression of the sodium/phosphate co-transporter Npt2c was sodium chloride sensitive. However, lack of tubular NHE3 blunted Npt2c expression. Alterations in the abundances of sodium/chloride cotransporter and its phosphorylation at threonine 58 as well as the abundances of the α-subunit of the epithelial sodium channel, and its cleaved form, were also apparent in NHE3loxloxCre mice. Thus, renal NHE3 is required to maintain blood pressure and steady-state plasma sodium levels when dietary sodium chloride intake is modified.

KEYWORDS:

aldosterone; distal tubule; hypernatremia; hyponatremia; proximal tubule

PMID:
28385297
PMCID:
PMC5511580
DOI:
10.1016/j.kint.2017.02.001
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for PubMed Central
Loading ...
Support Center