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J Vet Sci. 2017 Aug 31;18(S1):361-370. doi: 10.4142/jvs.2017.18.S1.361.

Generation, characterization, and application in serodiagnosis of recombinant swine vesicular disease virus-like particles.

Author information

1
National Centre for Foreign Animal Disease, Winnipeg, MB R3E 3M4, Canada.

Abstract

Swine vesicular disease (SVD) is a highly contagious viral disease that causes vesicular disease in pigs. The importance of the disease is due to its indistinguishable clinical signs from those of foot-and-mouth disease, which prevents international trade of swine and related products. SVD-specific antibody detection via an enzyme-linked immunosorbent assay (ELISA) is the most versatile and commonly used method for SVD surveillance and export certification. Inactivated SVD virus is the commonly used antigen in SVD-related ELISA. A recombinant SVD virus-like particle (VLP) was generated by using a Bac-to-Bac baculovirus expression system. Results of SVD-VLP analyses from electron microscopy, western blotting, immunofluorescent assay, and mass spectrometry showed that the recombinant SVD-VLP morphologically resemble authentic SVD viruses. The SVD-VLP was evaluated as a replacement for inactivated whole SVD virus in competitive and isotype-specific ELISAs for the detection of antibodies against SVD virus. The recombinant SVD-VLP assay produced results similar to those from inactivated whole virus antigen ELISA. The VLP-based ELISA results were comparable to those from the virus neutralization test for antibody detection in pigs experimentally inoculated with SVD virus. Use of the recombinant SVD-VLP is a safe and valuable alternative to using SVD virus antigen in diagnostic assays.

KEYWORDS:

enzyme-linked immunosorbent assay; serological tests; swine vesicular disease virus; virus-like particles

PMID:
28385002
PMCID:
PMC5583424
DOI:
10.4142/jvs.2017.18.S1.361
[Indexed for MEDLINE]
Free PMC Article

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