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Sci Rep. 2017 Apr 6;7:45816. doi: 10.1038/srep45816.

Investigating starch gelatinization through Stokes vector resolved second harmonic generation microscopy.

Author information

1
Institute of Biophotonics, National Yang-Ming University, Taipei, 11221, Taiwan. nirmaluva@gmail.com.
2
Department of Biophysics, School of Life Sciences, Manipal University, Manipal, 576104, India. nirmaluva@gmail.com.
3
Institute of Biophotonics, National Yang-Ming University, Taipei, 11221, Taiwan.
4
Key Laboratory of Biomedical Photonics, Huazhong University of Science and Technology, Wuhan, China.
5
Institute of Biophotonics, National Yang-Ming University, Taipei, 11221, Taiwan. fjkao@ym.edu.tw.

Abstract

The changes of the morphology during heating and the degree of crystallinity of dry and hydrated starch granules are investigated using second harmonic generation (SHG) based Stokes polarimetry. A spatial distribution of various polarization parameters, such as the degree of polarization (DOP), the degree of linear polarization (DOLP), and the degree of circular polarization (DOCP) are extracted and compared with the two dimensional second harmonic (SH) Stokes images of starch granules. The SH signal from hydrated and dry starch on heating differed significantly in DOLP and DOCP values, indicating that hydrated starch has a greater degree of ultrastructural amylopectin disorder. The detail of denaturation and the phase transition of hydrated starch demonstrate the significant influence of thermal processing.

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