Ca2+ Measurements in Mammalian Cells with Aequorin-based Probes

Bio Protoc. 2017 Mar 5;7(5):e2155. doi: 10.21769/BioProtoc.2155.

Abstract

Aequorin is a Ca2+ sensitive photoprotein suitable to measure intracellular Ca2+ transients in mammalian cells. Thanks to recombinant cDNAs expression, aequorin can be specifically targeted to various subcellular compartments, thus allowing an accurate measurement of Ca2+ uptake and release of different intracellular organelles. Here, we describe how to use this probe to measure cytosolic Ca2+ levels and mitochondrial Ca2+ uptake in mammalian cells.

Keywords: Aequorin; Ca2+; Luminescence; Probes.