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Plant Pathol J. 2017 Apr;33(2):193-205. doi: 10.5423/PPJ.OA.11.2016.0244. Epub 2017 Apr 1.

MoJMJ1, Encoding a Histone Demethylase Containing JmjC Domain, Is Required for Pathogenic Development of the Rice Blast Fungus, Magnaporthe oryzae.

Author information

1
Department of Agricultural Biotechnology, College of Agriculture and Life Science, Seoul National University, Seoul 08826, Korea.
2
Department of Biotechnology, College of Life and Applied Sciences, Yeungnam University, Gyeongsan 38541, Korea.
3
Department of Agricultural Biotechnology, College of Agriculture and Life Science, Seoul National University, Seoul 08826, Korea; Center for Fungal Genetic Resources and Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul 08826, Korea.

Abstract

Histone methylation plays important roles in regulating chromatin dynamics and transcription in eukaryotes. Implication of histone modifications in fungal pathogenesis is, however, beginning to emerge. Here, we report identification and functional analysis of a putative JmjC-domain-containing histone demethylase in Magnaporthe oryzae. Through bioinformatics analysis, we identified seven genes, which encode putative histone demethylases containing JmjC domain. Deletion of one gene, MoJMJ1, belonging to JARID group, resulted in defects in vegetative growth, asexual reproduction, appressorium formation as well as invasive growth in the fungus. Western blot analysis showed that global H3K4me3 level increased in the deletion mutant, compared to wild-type strain, indicating histone demethylase activity of MoJMJ1. Introduction of MoJMJ1 gene into ΔMojmj1 restored defects in pre-penetration developments including appressorium formation, indicating the importance of histone demethylation through MoJMJ1 during infection-specific morphogenesis. However, defects in penetration and invasive growth were not complemented. We discuss such incomplete complementation in detail here. Our work on MoJMJ1 provides insights into H3K4me3-mediated regulation of infection-specific development in the plant pathogenic fungus.

KEYWORDS:

Magnaporthe oryzae; histone demethylase; pathogenicity; rice blast

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