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PLoS Pathog. 2017 Apr 5;13(4):e1006305. doi: 10.1371/journal.ppat.1006305. eCollection 2017 Apr.

miR-144 attenuates the host response to influenza virus by targeting the TRAF6-IRF7 signaling axis.

Author information

1
Center for Infectious Disease Research, Seattle, WA United States of America.
2
Department of Comparative Medicine, School of Medicine, University of Washington, Seattle, WA, United States of America.
3
Institute for Systems Biology, Seattle, WA, United States of America.
4
Hematology, St. Jude Children's Research Hospital, Memphis, TN United States of America.

Abstract

Antiviral responses must rapidly defend against infection while minimizing inflammatory damage, but the mechanisms that regulate the magnitude of response within an infected cell are not well understood. miRNAs are small non-coding RNAs that suppress protein levels by binding target sequences on their cognate mRNA. Here, we identify miR-144 as a negative regulator of the host antiviral response. Ectopic expression of miR-144 resulted in increased replication of three RNA viruses in primary mouse lung epithelial cells: influenza virus, EMCV, and VSV. We identified the transcriptional network regulated by miR-144 and demonstrate that miR-144 post-transcriptionally suppresses TRAF6 levels. In vivo ablation of miR-144 reduced influenza virus replication in the lung and disease severity. These data suggest that miR-144 reduces the antiviral response by attenuating the TRAF6-IRF7 pathway to alter the cellular antiviral transcriptional landscape.

PMID:
28380049
PMCID:
PMC5393898
DOI:
10.1371/journal.ppat.1006305
[Indexed for MEDLINE]
Free PMC Article

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