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PLoS One. 2017 Apr 5;12(4):e0175033. doi: 10.1371/journal.pone.0175033. eCollection 2017.

Molecular cloning and expression analysis of the aqp1aa gene in half-smooth tongue sole (Cynoglossus semilaevis).

Guo H1,2, Wei M1,3, Liu Y1,3, Zhu Y1, Xu W1, Meng L1, Wang N1, Shao C1, Lu S1, Gao F1, Cui Z1,2, Wei Z1,2, Zhao F1,3,4, Chen S1,2,3,4.

Author information

1
Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences (CAFS), Key Laboratory for Sustainable Development of Marine Fisheries, Ministry of Agriculture, Qingdao, PR China.
2
College of Fisheries and Life, Shanghai Ocean University, Shanghai, PR China.
3
Wuxi Fisheries College, Nanjing Agricultural University, Wuxi, PR China.
4
Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China.

Abstract

Aquaporin 1 (AQP1) is a member of the transmembrane water channel family of proteins with special structural features, and two AQP1 paralogous genes (aqp1aa and aqp1ab) are reported in teleosts. In the present study, the aqp1aa gene of half-smooth tongue sole (Cynoglossus semilaevis) was cloned and characterized. The full-length cDNA of aqp1aa is 1411 bp with a 786 bp open reading frame encoding a 261-amino acid putative protein with a characteristic structure consisting of 6 membrane-spanning α-helical domains and two highly conserved asparagine-proline-alanine motifs. Real-time quantitative PCR revealed that aqp1aa mRNA is expressed predominantly in the testis of males and pseudo-males, while its expression is low in the ovary and lowest in doublesex and mab-3-related transcription factor 1(DMRT1) knock out fish and triploid males. In situ hybridization indicated that aqp1aa mRNA is expressed mainly in the germ cells of males and pseudo-males, especially in spermatozoa and spermatids. These results suggest that the aqp1aa may play a role in spermatogenesis of C. semilaevis.

PMID:
28380032
PMCID:
PMC5381947
DOI:
10.1371/journal.pone.0175033
[Indexed for MEDLINE]
Free PMC Article

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