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Ann Agric Environ Med. 2017 Mar 21;24(1):1-7. doi: 10.5604/12321966.1233891.

Division of methods for counting helminths' eggs and the problem of efficiency of these methods.

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Institute of Agrophysics, Polish Academy of Sciences, Doswiadczalna 4 Str., 20-290 Lublin; Poland.
Department of Biological Hazart and Parasitology, Institute of Rural Health, Jaczewskiego 2 Str., 20-090 Lublin, Poland.
Faculty of Environmental Engineering, Lublin University of Technology, Nadbystrzycka 40B Str., 20-618 Lublin; Poland.
Department of Parasitology and Invasive Diseases, National Veterinary Research Institute, Al. Partyzantow 57, 24-100 Pulawy, Poland.


From the sanitary and epidemiological aspects, information concerning the developmental forms of intestinal parasites, especially the eggs of helminths present in our environment in: water, soil, sandpits, sewage sludge, crops watered with wastewater are very important. The methods described in the relevant literature may be classified in various ways, primarily according to the methodology of the preparation of samples from environmental matrices prepared for analysis, and the sole methods of counting and chambers/instruments used for this purpose. In addition, there is a possibility to perform the classification of the research methods analyzed from the aspect of the method and time of identification of the individuals counted, or the necessity for staining them. Standard methods for identification of helminths' eggs from environmental matrices are usually characterized by low efficiency, i.e. from 30% to approximately 80%. The efficiency of the method applied may be measured in a dual way, either by using the method of internal standard or the 'Split/Spike' method. While measuring simultaneously in an examined object the efficiency of the method and the number of eggs, the 'actual' number of eggs may be calculated by multiplying the obtained value of the discovered eggs of helminths by inverse efficiency.

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