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Nucleic Acids Res. 1988 May 25;16(10):4447-63.

Cloning and characterization of a photolyase gene from the cyanobacterium Anacystis nidulans.

Author information

1
Research Institute for Tuberculosis and Cancer, Tohoku University, Sendai, Japan.

Abstract

A 2 kb fragment was isolated from an Anacystis nidulans genomic DNA library by hybridization with synthetic oligonucleotide probes derived from the N-terminal amino acid sequence of Anacystis photolyase. This fragment contains a 1452 bp-long open reading frame encoding a polypeptide of 484 amino acids (Mr 54475). Antibodies raised against purified Anacystis photolyase reacted with extracts of cells harboring fused genes between lacZ of Escherichia coli and this gene. A 40.7% similarity was found between the deduced amino acid sequences of Anacystis and E. coli photolyases, notwithstanding the difference in chromophore structure.

PMID:
2837735
PMCID:
PMC336641
DOI:
10.1093/nar/16.10.4447
[Indexed for MEDLINE]
Free PMC Article

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