Format

Send to

Choose Destination
Biochem Cell Biol. 2017 Aug;95(4):510-516. doi: 10.1139/bcb-2016-0255. Epub 2017 Apr 4.

Vitellogenin receptor selectively endocytoses female-specific and highly-expressed hemolymph proteins in the silkworm, Bombyx mori.

Han C1,1, Chen E1,1, Shen G1,1, Peng Z1,1, Xu Y1,1, Zhang H1,1, Liu H1,1, Zhang Y1,1, Wu J1,1, Lin Y1,1, Xia Q1,1.

Author information

1
State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, China.

Abstract

VgR, a member of the LDLR family, functions to transport vitellogenin into the ovaries to protome ovarian growth and embryonic development. In insects, the only widely accepted ligand of VgR is Vg. Recently, BmVgR has been shown to interact with BmSP1 in vitro. Therefore, in this study, we evaluated whether BmVgR could transport BmSP1 into certain cells. Although BmVgR could combine with BmVg and BmSP1, BmVgR did not affect the amount of BmSP1 taken up by Sf9 cells. Parallel immunofluorescence showed that most BmVg and BmVgR were localized in the inner oocyte membrane, showing tissue localization similar to that of BmVg labeled with pHrodo Red absorbed by the ovaries on day 2 of pupation. Although BmSP1 showed localization similar to BmVgR during the same phase, little BmSP1 was present in the ovary. Additionally, BmSP1 did not exist in ovaries when the ovaries contained BmVgR on day 5 of pupation, suggesting that BmSP1 in the ovaries was not endocytosed by BmVgR. In summary, BmVgR could facilitate uptake of BmVg by developing oocytes, but did not modulate in the transport of BmSP1.

KEYWORDS:

endocytose; endocytosis; protéine de stockage 1; récepteur de la vitellogénine; silkworm; storage protein 1; ver à soie; vitellogenin; vitellogenin receptor

PMID:
28376312
DOI:
10.1139/bcb-2016-0255
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Atypon
Loading ...
Support Center