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Front Microbiol. 2017 Mar 20;8:436. doi: 10.3389/fmicb.2017.00436. eCollection 2017.

Lactobacillus paraplantarum 11-1 Isolated from Rice Bran Pickles Activated Innate Immunity and Improved Survival in a Silkworm Bacterial Infection Model.

Author information

1
Genome Pharmaceuticals Institute Co. Ltd.Tokyo, Japan; Laboratory of Microbiology, Graduate School of Pharmaceutical Sciences, The University of TokyoTokyo, Japan; Department of Microbiology and Immunology, Teikyo University School of MedicineTokyo, Japan.
2
Genome Pharmaceuticals Institute Co. Ltd. Tokyo, Japan.
3
Teikyo University Institute of Medical Mycology Tokyo, Japan.
4
Department of Microbiology and Immunology, Teikyo University School of Medicine Tokyo, Japan.
5
Genome Pharmaceuticals Institute Co. Ltd.Tokyo, Japan; Laboratory of Microbiology, Graduate School of Pharmaceutical Sciences, The University of TokyoTokyo, Japan; Teikyo University Institute of Medical MycologyTokyo, Japan.

Abstract

Lactic acid bacteria (LAB) have high immune system-stimulating activity and are considered beneficial for human health as probiotics in the gut. The innate immune system is highly conserved between mammals and insects. Microbe-associated molecular patterns (e.g., peptidoglycan and β-glucan) induce cytokine maturation, which, in silkworm larvae, leads to muscle contraction. The purpose of this study is to find a novel probiotic by using silkworm muscle contraction assay. In the present study, we isolated LAB derived from rice bran pickles. We selected highly active LAB to activate the innate immune system of the silkworm, which was assayed based on silkworm muscle contraction. Of various LAB, L. paraplantarum 11-1 strongly stimulated innate immunity in the silkworm, leading to stronger silkworm contraction than a dairy-based LAB. Silkworms fed a diet containing L. paraplantarum 11-1 exhibited tolerance against the pathogenicity of Pseudomonas aeruginosa. These findings suggest that L. paraplantarum 11-1 could be a useful probiotic for activating innate immunity.

KEYWORDS:

Lactic acid bacteria; Lactobacillus sp.; Pseudomonas aeruginosa; infection; innate immunity; silkworm

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