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Anal Chim Acta. 2017 May 8;966:54-61. doi: 10.1016/j.aca.2017.02.011. Epub 2017 Feb 20.

Highly sensitive electrochemical immunoassay integrated with polymeric nanocomposites and enhanced SiO2@Au core-shell nanobioprobes for SirT1 determination.

Author information

1
College of Science, University of Shanghai for Science and Technology, Shanghai 200093, PR China; School of Chemistry and Molecular Engineering, East China Normal University, Shanghai 200241, PR China. Electronic address: anyarui@usst.edu.cn.
2
College of Science, University of Shanghai for Science and Technology, Shanghai 200093, PR China.
3
Zibo City Public Security, Zibo, 255000, PR China.
4
School of Chemistry and Molecular Engineering, East China Normal University, Shanghai 200241, PR China.
5
School of Chemistry and Molecular Engineering, East China Normal University, Shanghai 200241, PR China. Electronic address: zaxu@chem.ecnu.edu.cn.

Abstract

An ultra-highly sensitive electrochemical immunosensor for SirT1 (a key protein in age-related diseases) evaluation has been designed, employing polymeric nanocomposites as sensing platform and core-shell SiO2@Au to immobilize HRP-Ab2 as nanobioprobes. The approach includes chemical synthesis of PAMAM-Au-MWCNT nanocomposites (PNCs) with abundant PAMAM-Au nanoparticles immobilized on the MWCNT matrix, and biochemically synthesis of nanobioprobes with highly dispersed SiO2@Au tracing tags for successive efficient load functionalized enzyme-antibodies (HRP-Ab2). The PNCs nanocomposites could improve the efficiency of immune response via abundant capture antibodies (Ab1#1) on the electrode surface, and accelerate electron transfer through MWCNT and Au nanoparticles. Besides, the SiO2@Au was employed as tracing tags to label numerous HRP-Ab2 to further enhance signal readout during HRP-thionine-H2O2 system. Under optimal conditions, the signal intensity was linearly related to the concentration of SirT1 in the range of 20 pg mL-1 to 500 ng ml-1, and the limit of detection was 12.5 pg mL-1. It is noteworthy that the proposed immunoassay protocol has been successfully applied to evaluate SirT1 expression in cells by different treatment with high sensitivity and accuracy.

KEYWORDS:

Core-shell nanobioprobes; Electrochemistry; Immunoassay; Polymeric nanocomposites; SirT1

PMID:
28372727
DOI:
10.1016/j.aca.2017.02.011
[Indexed for MEDLINE]

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