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J Microbiol Biotechnol. 2017 Jun 28;27(6):1106-1111. doi: 10.4014/jmb.1611.11070.

Construction of Methanol-Sensing Escherichia coli by the Introduction of a Paracoccus denitrificans MxaY-Based Chimeric Two-Component System.

Author information

1
Department of Chemical Engineering, University of Ulsan, Ulsan 44610, Republic of Korea.
2
Research Center for Bio-based Chemistry, Korea Research Institute of Chemical Technology (KRICT), Ulsan 44610, Republic of Korea.
3
Department of Green Chemistry and Environmental Biotechnology, Korea University of Science and Technology (UST), Daejeon 34113, Republic of Korea.

Abstract

Escherichia coli was engineered to sense methanol by employing a chimeric two-component system (TCS) strategy. A chimeric MxaY/EnvZ (MxaYZ) TCS was constructed by fusing the Paracoccus denitrificans MxaY with the E. coli EnvZ. Real-time quantitative PCR analysis and GFP-based fluorescence analysis showed maximum transcription of ompC and the fluorescence at 0.01% of methanol, respectively. These results suggested that E. coli was successfully engineered to sense methanol by the introduction of chimeric MxaYZ. By using this strategy, various chimeric TCS-based bacterial biosensors can be constructed and used for the development of biochemical-producing recombinant microorganisms.

KEYWORDS:

Chimeric two-component system; Escherichia coli; green fluorescent protein; methanol

PMID:
28372037
DOI:
10.4014/jmb.1611.11070
[Indexed for MEDLINE]
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