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J Microbiol Biotechnol. 2017 Jun 28;27(6):1106-1111. doi: 10.4014/jmb.1611.11070.

Construction of Methanol-Sensing Escherichia coli by the Introduction of a Paracoccus denitrificans MxaY-Based Chimeric Two-Component System.

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Department of Chemical Engineering, University of Ulsan, Ulsan 44610, Republic of Korea.
Research Center for Bio-based Chemistry, Korea Research Institute of Chemical Technology (KRICT), Ulsan 44610, Republic of Korea.
Department of Green Chemistry and Environmental Biotechnology, Korea University of Science and Technology (UST), Daejeon 34113, Republic of Korea.


Escherichia coli was engineered to sense methanol by employing a chimeric two-component system (TCS) strategy. A chimeric MxaY/EnvZ (MxaYZ) TCS was constructed by fusing the Paracoccus denitrificans MxaY with the E. coli EnvZ. Real-time quantitative PCR analysis and GFP-based fluorescence analysis showed maximum transcription of ompC and the fluorescence at 0.01% of methanol, respectively. These results suggested that E. coli was successfully engineered to sense methanol by the introduction of chimeric MxaYZ. By using this strategy, various chimeric TCS-based bacterial biosensors can be constructed and used for the development of biochemical-producing recombinant microorganisms.


Chimeric two-component system; Escherichia coli; green fluorescent protein; methanol

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