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J Microbiol Biotechnol. 2017 Jun 28;27(6):1157-1162. doi: 10.4014/jmb.1702.02063.

Molecular Identification, Enzyme Assay, and Metabolic Profiling of Trichoderma spp.

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Department of Biotechnology, Yeungnam University, Gyeongsan 38541, Republic of Korea.
Department of Bioenergy Science and Technology, Chonnam National University, Gwangju 61186, Republic of Korea.


The goal of this study was to identify and characterize selected Trichoderma isolates by metabolic profiling and enzyme assay for evaluation of their potential as biocontrol agents against plant pathogens. Trichoderma isolates were obtained from the Rural Development Administration Genebank Information Center (Wanju, Republic of Korea). Eleven Trichoderma isolates were re-identified using ribosomal DNA internal transcribed spacer (ITS) regions. ITS sequence results showed new identification of Trichoderma isolates. In addition, metabolic profiling of the ethyl acetate extracts of the liquid cultures of five Trichoderma isolates that showed the best anti-Phytophthora activities was conducted using gas chromatography-mass spectrometry. Metabolic profiling revealed that Trichoderma isolates shared common metabolites with well-known antifungal activities. Enzyme assays indicated strong cell walldegrading enzyme activities of Trichoderma isolates. Overall, our results indicated that the selected Trichoderma isolates have great potential for use as biocontrol agents against plant pathogens.


Trichoderma; cell wall degradation enzyme activity; metabolic profiling; molecular identification

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