Format

Send to

Choose Destination
Vox Sang. 2017 May;112(4):326-335. doi: 10.1111/vox.12506. Epub 2017 Mar 31.

Metabolomics comparison of red cells stored in four additive solutions reveals differences in citrate anticoagulant permeability and metabolism.

Author information

1
Center for Systems Biology, University of Iceland, Reykjavik, Iceland.
2
Medical Department, University of Iceland, Reykjavik, Iceland.
3
The Blood Bank, Landspitali-University Hospital, Reykjavik, Iceland.
4
School of Science and Engineering, Reykjavik University, Reykjavik, Iceland.
5
Center for Biomedicine, European Academy of Bolzano/Bozen, Bolzano, Italy.
6
Sinopia Bioscience, San Diego, CA, USA.

Abstract

BACKGROUND AND OBJECTIVES:

Metabolomics studies have revealed transition points in metabolic signatures of red cells during storage in SAGM, whose clinical significance is unclear. We set out to investigate whether these transition points occur independent of storage media and define differences in the metabolism of red cells in additive solutions.

MATERIALS AND METHODS:

Red cell concentrates were stored in SAGM, AS-1, AS-3 or PAGGSM, and sampled fourteen times spanning Day 1-46. Following quality control, the samples were split into extracellular and intracellular aliquots. These were analysed with ultra-high-performance liquid chromatography coupled to mass spectrometry analysis affording quantitative metabolic profiles of both intra- and extracellular red cell metabolites.

RESULTS:

Differences were observed in glycolysis, purine salvage, glutathione synthesis and citrate metabolism on account of the storage solutions. Donor variability however hindered the accurate characterization of metabolic transition time-points. Intracellular citrate concentrations were increased in red cells stored in AS-3 and PAGGSM media. The metabolism of citrate in red cells in SAGM was subsequently confirmed using 13 C citrate isotope labelling and shown to originate from citrate anticoagulant.

CONCLUSION:

Metabolic signatures that discriminate between 'fresh' and 'old' stored red cells are dependent upon additive solutions. Specifically, the incorporation and metabolism of citrate in additive solutions with lower chloride ion concentration is altered and impacts glycolysis.

KEYWORDS:

SAGM; citrate; metabolomics; red cell storage; storage lesion

PMID:
28370161
DOI:
10.1111/vox.12506
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Wiley
Loading ...
Support Center