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Bioelectromagnetics. 2017 Jul;38(5):374-385. doi: 10.1002/bem.22049. Epub 2017 Mar 29.

Extremely low-frequency electromagnetic field exposure enhances inflammatory response and inhibits effect of antioxidant in RAW 264.7 cells.

Author information

1
College of Pharmacy, Chung-Ang University, Seoul, Republic of Korea.
2
Department of Pharmacology, College of Medicine, Chung-Ang University, Seoul, Republic of Korea.
3
Laboratory of Pharmacognosy, College of Pharmacy, Dankook University, Cheonan, Republic of Korea.

Abstract

In recent years, there has been a dramatic increase in the number and variety of electronic devices that emit electromagnetic waves. Because people live and work in close proximity to these pieces of electrical equipment, there is growing concern surrounding the destruction of homeostasis by electromagnetic field exposure. In the present study, the effects of 60 Hz 0.8 mT extremely low-frequency electromagnetic fields (ELF-EMF) on a macrophage cell line (RAW 264.7) were examined. Under defined ELF-EMF exposure conditions, the production of nitric oxide and pro-inflammatory cytokines, TNF-α, IL-1β, and IL-6, were increased in RAW 264.7 cells and the expression of those genes was also upregulated. However, cell proliferation was not altered. Translocation of NF-κB (nuclear factor kappa B), molecules that act downstream of the pro-inflammatory cytokines, were increased to the nucleus under ELF-EMF exposure conditions. In addition, we found that ELF-EMF exposure elevated activation of nuclear factor of activated T cells (NFAT) 2, as well as positively affected the influx of calcium. Furthermore, with both the presence of a potent antioxidant (Resveratrol) and downregulation of the antioxidant-related gene Prx-1 (Peroxiredoxin-1), ELF-EMF was associated with higher inflammatory responses of macrophages. These results suggest that an ELF-EMF amplifies inflammatory responses through enhanced macrophage activation and can decrease the effectiveness of antioxidants. Bioelectromagnetics. 38:374-385, 2017.

KEYWORDS:

ELF-EMF; inflammatory cytokine; macrophage; peroxiredoxin-1; resveratrol

PMID:
28370033
DOI:
10.1002/bem.22049
[Indexed for MEDLINE]

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