Format

Send to

Choose Destination
J Cell Physiol. 2018 Feb;233(2):946-957. doi: 10.1002/jcp.25938. Epub 2017 May 23.

Connexin43 intercellular communication drives the early differentiation of human bone marrow stromal cells into osteoblasts.

Talbot J1, Brion R1,2,3,4, Lamora A1,2,3,4, Mullard M1,2,3, Morice S1,2,3, Heymann D1,4,5, Verrecchia F1,2,3.

Author information

1
INSERM, UMR 957, Nantes, France.
2
INSERM, UMR 1238, Nantes, France.
3
Université Bretagne Loire, Nantes, France.
4
CHU, Nantes, France.
5
Department of Oncology and metabolism, INSERM European Associated Laboratory, University of Sheffield, Shefflied, UK.

Abstract

Although it has been demonstrated that human bone marrow stromal cells (hBMSCs) express the ubiquitous connexin43 (Cx43) and form functional gap junctions, their role in the early differentiation of hBMSCs into osteoblasts remains poorly documented. Using in vitro assays, we show that Cx43 expression and gap junctional intercellular communication (GJIC) are increased during the differentiation of hBMSCs into osteoblasts, both at the protein and mRNA levels. Two independent procedures to reduce GJIC, a pharmacological approach with GJIC inhibitors (18α-glycyrrhetinic acid and Gap27 peptide) and a molecular approach using small interfering RNA against Cx43, demonstrated that the presence of Cx43 and functional junctional channels are essential to the ability of hBMSCs to differentiate into osteoblasts in vitro. In addition, a reduced GJIC decreases the expression of Runx2, the major transcription factor implicated in the control of osteoblast commitment and early differentiation of hBMSCs into osteoblasts, suggesting that GJIC mediated by Cx43 is implicated in this process. Together our results demonstrate that GJIC mediated by the Cx43 channels plays a central role throughout the differentiation of hBMSC into osteoblasts, from the early stages to the process of mineralization.

KEYWORDS:

cell differentiation; connexin43; gap junction; human bone marrow stromal cells; intercellular communication; osteoblast

PMID:
28369869
DOI:
10.1002/jcp.25938
[Indexed for MEDLINE]
Free full text

Supplemental Content

Full text links

Icon for Wiley Icon for White Rose Research Online
Loading ...
Support Center