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J Gerontol A Biol Sci Med Sci. 2017 Aug 1;72(8):1033-1037. doi: 10.1093/gerona/glx017.

Environmental Canalization of Life Span and Gene Expression in Caenorhabditis elegans.

Author information

1
Department of Pathology, University of Washington, Seattle.
2
Department of Molecular and Integrative Physiology, University of Michigan, Ann Arbor.
3
Institute for Behavioral Genetics.
4
Department of Integrative Physiology.
5
Biofrontiers Institute, University of Colorado, Boulder.
6
Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, Washington.

Abstract

Animals, particularly poikilotherms, exhibit distinct physiologies at different environmental temperatures. Here, we hypothesized that temperature-based differences in physiology could affect the amount of variation in complex quantitative traits. Specifically, we examined, in Caenorhabditis elegans, how different temperatures (15°C, 20°C, and 25°C) affected the amount of interindividual variation in life span and also expression of three reporter genes-transcriptional reporters for vit-2, gpd-2, and hsp-16.2 (a life-span biomarker). We found the expected inverse relationship between temperature and average life span. Surprisingly, we found that at the highest temperature, there were fewer differences between individuals in life span and less interindividual variation in expression of all three reporters. We suggest that growth at 25°C might canalize (reduce interindividual differences in) life span and expression of some genes by eliciting a small constitutive heat shock response. Growth at 25°C requires wild-type hsf-1, which encodes the main heat shock response transcriptional activator. We speculate that increased chaperone activity at 25°C may reduce interindividual variation in gene expression by increasing protein folding efficiency. We hypothesize that reduced variation in gene expression may ultimately cause reduced variation in life span.

KEYWORDS:

Aging; Biomarker; Nongenetic; Proteostasis; Variation

PMID:
28369388
PMCID:
PMC5861850
DOI:
10.1093/gerona/glx017
[Indexed for MEDLINE]
Free PMC Article

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