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Gigascience. 2017 Jan 1;6(1):1-9. doi: 10.1093/gigascience/giw004.

Genome sequence of a rice pest, the white-backed planthopper (Sogatella furcifera).

Author information

1
School of Life Sciences, University of Science and Technology of China, Hefei, Anhui 230027, China.
2
Guangdong Province Key Laboratory of Microbial Signals and Disease Control, College of Agriculture, South China Agricultural University, Guangzhou, Guangdong 510642, China.
3
BGI-Shenzhen, Beishan Industrial Zone, Yantian District, Shenzhen 518083, China.
4
Chinese Academy of Sciences Key Laboratory of Innate Immunity and Chronic Disease, University of Science and Technology of China, Hefei, Anhui 230027, China.
5
Hefei National Laboratory for Physical Sciences at the Microscale, Bio-X Interdisciplinary Sciences, 443 Huang-Shan Road, Hefei, Anhui 230027, China.

Abstract

Background:

Sogatella furcifera is an important phloem sap-sucking and plant virus-transmitting migratory insect of rice. Because of its high reproductive potential, dispersal capability and transmission of plant viral diseases, S. furcifera causes considerable damage to rice grain production and has great economical and agricultural impacts. Comprehensive studies into ecological aspects and virus-host interactions of S. furcifera have been limited because of the lack of a well-assembled genome sequence.

Findings:

A total of 241.3 Gb of raw reads from the whole genome of S. furcifera were generated by Illumina sequencing using different combinations of mate-pair and paired-end libraries from 17 insert libraries ranging between 180 bp and 40 kbp. The final genome assembly (0.72 Gb), with average N50 contig size of 70.7 kb and scaffold N50 of 1.18 Mb, covers 98.6 % of the estimated genome size of S. furcifera . Genome annotation, assisted by eight different developmental stages (embryos, 1 st -5 th instar nymphs, 5-day-old adults and 10-day-old adults), generated 21 254 protein-coding genes, which captured 99.59 % (247/248) of core CEGMA genes and 91.7 % (2453/2675) of BUSCO genes.

Conclusions:

We report the first assembled and annotated whole genome sequence and transcriptome of S. furcifera . The assembled draft genome of S. furcifera will be a valuable resource for ecological and virus-host interaction studies of this pest.

KEYWORDS:

Annotation; Assembly; Genomics; Sogatella furcifera genome

PMID:
28369349
PMCID:
PMC5437944
DOI:
10.1093/gigascience/giw004
[Indexed for MEDLINE]
Free PMC Article

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