Format

Send to

Choose Destination
Immunol Lett. 2017 Jun;186:33-40. doi: 10.1016/j.imlet.2017.03.016. Epub 2017 Mar 31.

Recombinant Mip-PilE-FlaA dominant epitopes vaccine candidate against Legionella pneumophila.

Author information

1
Department of Parasitology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu, China.
2
First Department of Hepatobiliary Surgery, Qinghai University Affiliated Hospital, Medical College of Qinghai University, Xining, China.
3
Department of Parasitology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu, China; Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, Chengdu, China. Electronic address: jpchen007@163.com.

Abstract

Legionella pneumophila is the main causative agent of Legionnaires' disease, which is a severe multi-system disease with pneumonia as the primary manifestation. We designed a recombinant Mip-PilE-FlaA dominant epitopes vaccine against Legionella pneumophila to prevent the disease and evaluated its immunogenicity and protective immunity. The protein structures of Mip, PilE and FlaA were analyzed using a computer, and the gene sequences of the dominant epitopes of the three proteins were selected to construct and optimize the vaccine. The optimized mip, pilE, flaA and recombinant mip-pilE-flaA gene sequences were cloned, expressed and purified. The purified proteins were used as dominant epitopes vaccines to immunize BALB/c mice and determine the protective immunity and immunogenicity of these purified proteins. The identification confirmed that the recombinant mip-pilE-flaA was successfully cloned and expressed. ELISA revealed that the Mip-PilE-FlaA group produced the highest IgG response, and this protein may considerably improve the production of some cytokines in BALB/c mice. Histopathology analyses of lungs from mice immunized with Mip-PilE-FlaA revealed a certain protective effect. Our work demonstrated that the recombinant dominant epitopes of Mip-PilE-FlaA exhibited strong immunogenicity and immune protection, and this protein may be an efficient epitopes vaccine candidate against Legionella pneumophila.

KEYWORDS:

Epitopes vaccine; FlaA; Immunity; Legionella pneumophila; Mip; PilE

PMID:
28366526
DOI:
10.1016/j.imlet.2017.03.016
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center