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Cell Physiol Biochem. 2017;41(5):1736-1752. doi: 10.1159/000471866. Epub 2017 Mar 31.

Bone Marrow-Derived Mononuclear Cell Therapy Accelerates Renal Ischemia-Reperfusion Injury Recovery by Modulating Inflammatory, Antioxidant and Apoptotic Related Molecules.

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Laboratory of Cellular and Molecular Physiology, Rio de Janeiro, Brazil.
Laboratory of Immunopathology, Rio de Janeiro, Brazil.
Laboratory for Clinical and Experimental Research on Vascular Biology, Biomedical Center, State University of Rio de Janeiro, Rio de Janeiro, Brazil.
Confocal Microscopy Unit, Institute of Biomedical Science, Rio de Janeiro, Brazil.
Laboratory of Pulmonary Investigation, Institute of Biophysics Carlos Chagas Filho, Rio de Janeiro, Brazil.
Department of Radiology, School of Medicine, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.



We investigated the regenerative capacity of intravenous administration of bone marrow-derived mononuclear cells (BMMCs) in a rat model of bilateral renal ischemia/reperfusion (IR) injury and the involvement of inflammatory anti-inflammatory and other biological markers in this process.


Rats were subjected to 1h bilateral renal pedicle clamping. BMMCs were injected i.v 1h after reperfusion and tracked by 99mTc and GFP+ BMMCs. Twenty-four hours after reperfusion, renal function and histological changes were evaluated. The mRNA (real time PCR) and protein (ELISA and immuno-staining) expression of biological markers were analyzed.


Renal function and structure improved after infusion of BMMCs in the IR group (IR-C). Labeled BMMCs were found in the kidneys after therapy. The expression of inflammatory and biological markers (TLR-2, TRL-4, RAGE, IL-17, HMGB-1, KIM-1) were reduced and the expression of anti-inflammatory and antioxidant markers (IL-10, Nrf2, and HO-1) were increased in IR-C animals compared with IR untreated animals (IR-S). The apoptotic index diminished and the proliferation index increased in IR-C compared with IR-S.


The results contribute to our understanding of the role of different biological players in morphofunctional renal improvement and cytoprotection in a post-ischemic reperfusion kidney injury model subjected to cellular therapy.


Bone marrow; Cellular therapy; Ischemia- reperfusion; Renal

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