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Immunogenetics. 2017 Jun;69(6):401-407. doi: 10.1007/s00251-017-0982-x. Epub 2017 Mar 31.

The scavenging capacity of DMBT1 is impaired by germline deletions.

Author information

1
Department of Oral Biochemistry, Academic Centre for Dentistry Amsterdam, University of Amsterdam and VU University Amsterdam, Gustav Mahlerlaan 3004, 1081LA, Amsterdam, Netherlands. fbikker@acta.nl.
2
Division of Molecular Genome Analysis, German Cancer Research Center, Heidelberg, Germany.
3
Department of Oral Biochemistry, Academic Centre for Dentistry Amsterdam, University of Amsterdam and VU University Amsterdam, Gustav Mahlerlaan 3004, 1081LA, Amsterdam, Netherlands.
4
Molecular Oncology, University of Southern Denmark, Odense, Denmark.

Abstract

The Scavenger Receptor Cysteine-Rich (SRCR) proteins are an archaic group of proteins characterized by the presence of multiple SRCR domains. They are membrane-bound or secreted proteins, which are generally related to host defense systems in animals. Deleted in Malignant Brain Tumors 1 (DMBT1) is a SRCR protein which is secreted in mucosal fluids and involved in host defense by pathogen binding by its SRCR domains. Genetic polymorphism within DMBT1 leads to DMBT1-alleles giving rise to polypeptides with interindividually different numbers of SRCR domains, ranging from 8 SRCR domains (encoded by 6 kb DMBT1 variant) to 13 SRCR domains (encoded by the 8 kb DMBT1 variant). In the present study, we have investigated whether reduction from 13 to 8 amino-terminal SRCR domains leads to reduction of bacterial binding. The 6 kb variant bound ~20-45% less bacteria compared to the 8 kb variant. These results support the hypothesis that genetic variation in DMBT1 may influence microbial defense.

KEYWORDS:

Genetic polymorphism; Microbial defense; Pathogen binding; SRCR domain

PMID:
28364129
PMCID:
PMC5435793
DOI:
10.1007/s00251-017-0982-x
[Indexed for MEDLINE]
Free PMC Article

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