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Nat Methods. 2017 Mar 31;14(4):374-380. doi: 10.1038/nmeth.4218.

Adaptive optical fluorescence microscopy.

Author information

1
Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia, USA.

Abstract

The past quarter century has witnessed rapid developments of fluorescence microscopy techniques that enable structural and functional imaging of biological specimens at unprecedented depth and resolution. The performance of these methods in multicellular organisms, however, is degraded by sample-induced optical aberrations. Here I review recent work on incorporating adaptive optics, a technology originally applied in astronomical telescopes to combat atmospheric aberrations, to improve image quality of fluorescence microscopy for biological imaging.

PMID:
28362438
DOI:
10.1038/nmeth.4218
[Indexed for MEDLINE]

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