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Hemoglobin. 2016 Nov;40(6):417-419. doi: 10.1080/03630269.2016.1276930.

Detection of the Unstable Hb Köln (HBB: c.295G>A) by a Capillary Electrophoresis Method.

Author information

1
a The Center of Reproduction and Genetic , People's Hospital of Guangxi Zhuang Autonomous Region , Nanning , Guangxi , People's Republic of China.
2
b Department of Clinical Laboratory , Laibing Maternal and Neonatal Hospital , Laibing , Guangxi , People's Republic of China.
3
c Department of Obstetrics and Gynecology , People's Hospital of Guangxi Zhuang Autonomous Region , Nanning , Guangxi , People's Republic of China.

Abstract

Hb Köln (HBB: c.295G>A) is an unstable β-globin gene variant with a GTG>ATG substitution at codon 98. This variant is quite frequent in Europe and the USA but rare in China. It can easily be misdiagnosed as Hb Constant Spring (Hb CS; HBA2: c.427T>C) by high performance liquid chromatography (HPLC), but detection and quantification of both Hb Köln and degraded Hb Köln by capillary electrophoresis (CE) are possible. Thus, we concluded that CE was the preferred method for Hb Köln detection.

KEYWORDS:

Capillary electrophoresis; Hb Köln; high performance liquid chromatography

PMID:
28361589
DOI:
10.1080/03630269.2016.1276930
[Indexed for MEDLINE]

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