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Methods Mol Biol. 2017;1597:229-245. doi: 10.1007/978-1-4939-6949-4_17.

In Vivo Model of Small Intestine.

Author information

1
Department of Pediatric General and Thoracic Surgery, Cincinnati Children's Hospital Medical Center, 3333 Burnet Avenue, Cincinnati, OH, 45229, USA.
2
Department of Pediatrics, University of Cincinnati, Cincinnati, OH, USA.
3
Department of Pediatric General and Thoracic Surgery, Cincinnati Children's Hospital Medical Center, 3333 Burnet Avenue, Cincinnati, OH, 45229, USA. michael.helmrath@cchmc.org.
4
Department of Pediatrics, University of Cincinnati, Cincinnati, OH, USA. michael.helmrath@cchmc.org.

Erratum in

Abstract

The utilization of human pluripotent stem cells (hPSCs) offers new avenues in the generation of organs and opportunities to understand development and diseases. The hPSC-derived human intestinal organoids (HIOs) provide a new tool to gain insights in small intestinal development, physiology, and associated diseases. Herein, we provide a method for orthotropic transplantation of HIOs in immunocompromised mice. This method highlights the specific steps to successful engraftment and provides insight into the study of bioengineered human small intestine.

KEYWORDS:

Bioengineered small intestine; Human intestinal organoids; In vivo model; Kidney capsule; Mesentery; Pluripotent stem cells; Transplantation

PMID:
28361322
PMCID:
PMC5559894
DOI:
10.1007/978-1-4939-6949-4_17
[Indexed for MEDLINE]
Free PMC Article

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