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Lipids Health Dis. 2017 Mar 31;16(1):66. doi: 10.1186/s12944-017-0458-x.

17β-estradiol regulates the expression of apolipoprotein M through estrogen receptor α-specific binding motif in its promoter.

Author information

1
Department of Comprehensive Laboratory, the Third Affiliated Hospital of Soochow University, Changzhou, 213003, China.
2
Division of Clinical Chemistry and Pharmacology, Department of Laboratory Medicine, Lunds University, S-221 85, Lund, Sweden.
3
Cardiothoracic Surgery, the Third Affiliated Hospital of Soochow University, Changzhou, 213003, China.
4
Division of Clinical Chemistry and Pharmacology, Department of Laboratory Medicine, Lunds University, S-221 85, Lund, Sweden. ning.xu@med.lu.se.

Abstract

BACKGROUND:

We have previously demonstrated that estrogen could significantly enhance expression of apolipoprotein M (apoM), whereas the molecular basis of its mechanism is not fully elucidated yet. To further investigate the mechanism behind the estrogen induced up-regulation of apoM expression.

RESULTS:

Our results demonstrated either free 17β-estradiol (E2) or membrane-impermeable bovine serum albumin-conjugated E2 (E2-BSA) could modulate human apoM gene expression via the estrogen receptor alpha (ER-α) pathway in the HepG2 cells. Moreover, experiments with the luciferase activity analysis of truncated apoM promoters could demonstrate that a regulatory region (from-1580 to -1575 bp (-GGTCA-)) upstream of the transcriptional start site of apoM gene was essential for the basal transcriptional activity that regulated by the ER-α. With the applications of an electrophoresis mobility shift assay and a chromatin immunoprecipitation assay, we could successfully identify a specific ER-α binding element in the apoM promoter region.

CONCULSION:

In summary, the present study indicates that 17β-estradiol induced up-regulation of apoM in HepG2 cells is through an ER-α-dependent pathway involving ER-α binding element in the promoter of the apoM gene.

KEYWORDS:

Apolipoprotein M; Chromatin Immunoprecipitation (ChIP) assay; Electrophoretic mobility shift assay (EMSA); Estrogen receptor alpha; Estrogen responsive element

PMID:
28359281
PMCID:
PMC5374570
DOI:
10.1186/s12944-017-0458-x
[Indexed for MEDLINE]
Free PMC Article

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