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J Med Biochem. 2015 Apr;34(2):179-190. doi: 10.2478/jomb-2014-0035. Epub 2015 Mar 3.

Post-Effort Changes in Activity of Traditional Diagnostic Enzymatic Markers in Football Players' Blood.

Author information

1
Faculty of Sport Education, Gdansk University of Physical Education and Sport, Gdansk, Poland.
2
Institute of Sports, University School of Physical Education in Cracow, Cracow, Poland.
3
Department of Biochemistry, University of Szczecin, Szczecin, Poland.
4
Faculty of Physical Education and Health Promotion, University of Szczecin, Szczecin, Poland.
5
Faculty of Sport Education, Gdansk University of Physical Education and Sport, Gdansk, Poland; Faculty of Physical Education and Health Promotion, University of Szczecin, Szczecin, Poland.

Abstract

in English, Serbian

BACKGROUND:

Long-term and intensive physical effort causes metabolic and biochemical adaptations for both athletic and non-athletic objectives. Knowing the importance of aerobic training in football players, the aim of this study was to evaluate changes in the activity of: creatinine kinase (CK), creatine kinase MB (CKMB), lactate dehydrogenase (LDH), α-hydroxybutyrate dehydrogenase (HBDH), cholinesterase (ChE) and alkaline phosphatase (ALP) in response to a semi-long distance outdoor run under aerobic conditions among both female and male football players.

METHODS:

Sixteen participants aged 21.9±2 years (women) and 18.4±0.5 years (men), all of them voluntarily recruited football players, took part in an outdoor run, the women covering a distance of 7.4±0.3 km while men covered a distance of 10.7±1.0 km. Plasma activities of the studied enzymes were determined using an appropriate diagnostic assay kit.

RESULTS:

Our results indicate that total LDH activity could be a useful tool in evaluating physical fitness among athletes. We simultaneously established that ChE could not be a marker useful in assessing metabolic response to physical effort in athletes. Moreover, our results suggest that post-effort changes in ALP activity might be used to estimate early symptoms of certain vitamin deficiencies in an athlete's diet.

CONCLUSIONS:

We confirmed that the assessment of activity of selected traditional diagnostic enzymatic markers provides information about muscle state after physical effort.

KEYWORDS:

aerobic training; athletes; enzymatic markers

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