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J Pharmacol Exp Ther. 2017 Jun;361(3):408-416. doi: 10.1124/jpet.116.238113. Epub 2017 Mar 29.

Soluble Epoxide Hydrolase Pharmacological Inhibition Decreases Alveolar Bone Loss by Modulating Host Inflammatory Response, RANK-Related Signaling, Endoplasmic Reticulum Stress, and Apoptosis.

Author information

1
Department of Entomology and Nematology and University of California, Davis Comprehensive Cancer Center (C.A.T.-S., K.S.S.L., B.I., S.K.G., B.D.H.), Nutrition Department (F.G.H.), and Department of Molecular Biosciences, School of Veterinary Medicine (D.B.), University of California, Davis, California; Institute of Genetics and Biochemistry, Federal University of Uberlândia, Uberlândia, Brazil (C.A.T.-d.-S., C.U.-V.); Laboratory of Immunology and Molecular Biology, São Leopoldo Mandic Institute and Research Center, Campinas, Brazil (M.H.N.); and Department of Nutrition, University of Tennessee-Knoxville, Knoxville, Tennessee (A.B.).
2
Department of Entomology and Nematology and University of California, Davis Comprehensive Cancer Center (C.A.T.-S., K.S.S.L., B.I., S.K.G., B.D.H.), Nutrition Department (F.G.H.), and Department of Molecular Biosciences, School of Veterinary Medicine (D.B.), University of California, Davis, California; Institute of Genetics and Biochemistry, Federal University of Uberlândia, Uberlândia, Brazil (C.A.T.-d.-S., C.U.-V.); Laboratory of Immunology and Molecular Biology, São Leopoldo Mandic Institute and Research Center, Campinas, Brazil (M.H.N.); and Department of Nutrition, University of Tennessee-Knoxville, Knoxville, Tennessee (A.B.) bdhammock@ucdavis.edu.

Abstract

Epoxyeicosatrienoic acids (EETs), metabolites of arachidonic acid derived from the cytochrome P450 enzymes, are mainly metabolized by soluble epoxide hydrolase (sEH) to their corresponding diols. EETs but not their diols, have anti-inflammatory properties, and inhibition of sEH might provide protective effects against inflammatory bone loss. Thus, in the present study, we tested the selective sEH inhibitor, 1-trifluoromethoxyphenyl-3-(1-propionylpiperidin-4-yl) urea (TPPU), in a mouse model of periodontitis induced by infection with Aggregatibacter actinomycetemcomitans Oral treatment of wild-type mice with TPPU and sEH knockout (KO) animals showed reduced bone loss induced by A. actinomycetemcomitans This was associated with decreased expression of key osteoclastogenic molecules, receptor activator of nuclear factor-κB/RANK ligand/osteoprotegerin, and the chemokine monocyte chemotactic protein 1 in the gingival tissue without affecting bacterial counts. In addition, downstream kinases p38 and c-Jun N-terminal kinase known to be activated in response to inflammatory signals were abrogated after TPPU treatment or in sEH KO mice. Moreover, endoplasmic reticulum stress was elevated in periodontal disease but was abrogated after TPPU treatment and in sEH knockout mice. Together, these results demonstrated that sEH pharmacological inhibition may be of therapeutic value in periodontitis.

PMID:
28356494
PMCID:
PMC5443319
DOI:
10.1124/jpet.116.238113
[Indexed for MEDLINE]
Free PMC Article

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