Format

Send to

Choose Destination
Anticancer Agents Med Chem. 2017 Nov 24;17(11):1519-1525. doi: 10.2174/1871520617666170327152026.

Anti-cancer Effect of Cyanidin-3-glucoside from Mulberry via Caspase-3 Cleavage and DNA Fragmentation in vitro and in vivo.

Author information

1
Department of Biochemistry and Institute of Cardiovascular Research, Chonbuk National University Medical School, Jeonju, 54896, Korea
2
Department of Anesthesiology and Pain Medicine,Bucheon St. Mary’s Hospital, the Catholic University of Korea, Bucheon 14647, Korea
3
Department of Obstetrics and Gynecology, Chonbuk National University Medical School, Jeonju, 54896, Korea
4
Department of Companion and Laboratory Animal Science, Konju National University, Yesan, 32439, Korea
5
Department of Life Science and Graduate School of Energy Storage/Conversion Engineering, Chonbuk National University, Jeonju, 54896, Korea
6
Department of Oriental Pharmaceutical Development, Nambu University, Gwangju, 62271, Korea

Abstract

BACKGROUND:

Fruits of Morus alba L. (mulberry) have various bioactive compounds such as polyphenols and anthocyanins and used as a herbal medicine. However, the anti-cancer effects and molecular basis have not been elucidated.

METHODS:

We isolated the cyanidin-3-glucoside in various cultivar of mulberry by acidified-methanol extraction methods. This molecule were compared mass spectroscopic properties by LC-MS/MS and analyzed by 1H and 13C NMR. We examined the anti-cancer effect with molecular mechanisms of the cyanidin-3-glucoside on MDA-MB-453 human breast cancer cells and xenograft animal model.

RESULTS:

The treatment with the mulberry cyanidin-3-glucoside decreased cell viability in a dose-dependent manner with alteration of apoptotic protein contents, and DNA fragmentation, suggesting that cells undergo apoptosis. Supporting the observations, Treatment with the cyanidin-3-glucoside showed active apoptosis by caspase-3 cleavage and DNA fragmentation through Bcl-2 and Bax pathway. Indeed, cyanidin-3-glucoside inhibits tumor growth in MDA-MB-453 cells-inoculated nude mice. Tumor growth of xenograft nude mouse was significantly reduced compared to the control group by the cyanidin-3-glucoside.

CONCLUSION:

The data demonstrate that cyanidin-3-glucoside isolated from mulberry induced apoptosis in breast cancer (MDA-MB-453) cells, and therefore, has a potential as an anti-cancer agent. These results show that mulberry cyanidin-3-glucoside inhibit the proliferation and growth in vitro and in vivo model and, indicating the inhibition of tumor progression.

KEYWORDS:

Cyanidin-3-glucoside; DNA fragmentation; Morus alba L.; anti-cancer; caspase-3; mulberry; nude mice.

[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Bentham Science Publishers Ltd.
Loading ...
Support Center