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Methods Mol Biol. 2017;1590:199-206. doi: 10.1007/978-1-4939-6921-0_15.

Culturing and Cryobanking Human Neural Stem Cells.

Author information

1
ARC Centre of Excellence for Electromaterials Science, Intelligent Polymer Research Institute, AIIM Facility, Innovation Campus, University of Wollongong, Fairy Meadow, New South Wales, 2519, Australia. jcrook@uow.edu.au.
2
Illawarra Health and Medical Research Institute, University of Wollongong, Wollongong, New South Wales, 2522, Australia. jcrook@uow.edu.au.
3
Department of Surgery, St. Vincent's Hospital, The University of Melbourne, Fitzroy, Victoria, 3065, Australia. jcrook@uow.edu.au.
4
ARC Centre of Excellence for Electromaterials Science, Intelligent Polymer Research Institute, AIIM Facility, Innovation Campus, University of Wollongong, Fairy Meadow, New South Wales, 2519, Australia.
5
Illawarra Health and Medical Research Institute, University of Wollongong, Wollongong, New South Wales, 2522, Australia.

Abstract

The discovery and study of human neural stem cells has advanced our understanding of human neurogenesis, and the development of novel therapeutics based on neural cell replacement. Here, we describe methods to culture and cryopreserve human neural stem cells (hNSCs) for expansion and banking. Importantly, the protocols ensure that the multipotency of hNSCs is preserved to enable differentiation to neurons and supporting neuroglia.

KEYWORDS:

Cryopreservation; Culture; Expansion; Freezing; Human neural stem cells; Passaging

PMID:
28353272
DOI:
10.1007/978-1-4939-6921-0_15
[Indexed for MEDLINE]

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