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Methods Mol Biol. 2017;1590:151-164. doi: 10.1007/978-1-4939-6921-0_11.

Cryobanking Pluripotent Stem Cells.

Author information

1
ARC Centre of Excellence for Electromaterials Science, Intelligent Polymer Research Institute, AIIM Facility, Innovation Campus, University of Wollongong, Fairy Meadow, New South Wales, 2519, Australia. jcrook@uow.edu.au.
2
Illawarra Health and Medical Research Institute, University of Wollongong, Wollongong, New South Wales, 2522, Australia. jcrook@uow.edu.au.
3
Department of Surgery, St Vincent's Hospital, The University of Melbourne, Fitzroy, Victoria, 3065, Australia. jcrook@uow.edu.au.
4
ARC Centre of Excellence for Electromaterials Science, Intelligent Polymer Research Institute, AIIM Facility, Innovation Campus, University of Wollongong, Fairy Meadow, New South Wales, 2519, Australia.
5
Illawarra Health and Medical Research Institute, University of Wollongong, Wollongong, New South Wales, 2522, Australia.
6
WiCell Research Institute, Madison, WI, USA.

Abstract

Cryobanking human pluripotent stem cells (hPSCs), be they human embryonic (hESCs) or induced pluripotent stem cells (iPSCs), is essential for their use in research and cell-based therapeutics. Working and master cell banks can be generated with a desired level of quality assurance applied during cell freezing and storage. Conventional vitrification has evolved to more advanced control rate freezing, culminating in a myriad of published protocols with variable proficiencies and clinical efficacies. Notwithstanding, standardized and reliable protocols are necessary for basic science through to applied research and clinical product development. This chapter details several methods for hPSC cryopreservation, suitable for routine application, high-quality research, and adaptable for clinical compliance.

KEYWORDS:

Banking; Control rate freezing; Cryopreservation; Human pluripotent stem cells; Vitrification

PMID:
28353268
DOI:
10.1007/978-1-4939-6921-0_11
[Indexed for MEDLINE]

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