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Sci Rep. 2017 Mar 28;7(1):482. doi: 10.1038/s41598-017-00578-x.

Rapid generation of a transgene-free powdery mildew resistant tomato by genome deletion.

Author information

1
The Sainsbury Laboratory, Norwich Research Park, Norwich, NR4 7UH, UK.
2
Rothamsted Research, Harpenden, Hertfordshire, AL5 2JQ, UK.
3
Institute of Digital Agriculture, Zhejiang Academy of Agricultural Sciences, Hangzhou, Zhejiang, 310021, P.R. China.
4
Department of Molecular Biology, Max Planck Institute for Developmental Biology, 72076, Tübingen, Germany.
5
Department of Molecular Biology, Max Planck Institute for Developmental Biology, 72076, Tübingen, Germany. detlef.weigel@tuebingen.mpg.de.
6
The Sainsbury Laboratory, Norwich Research Park, Norwich, NR4 7UH, UK. sophien.kamoun@tsl.ac.uk.

Abstract

Genome editing has emerged as a technology with a potential to revolutionize plant breeding. In this study, we report on generating, in less than ten months, Tomelo, a non-transgenic tomato variety resistant to the powdery mildew fungal pathogen using the CRISPR/Cas9 technology. We used whole-genome sequencing to show that Tomelo does not carry any foreign DNA sequences but only carries a deletion that is indistinguishable from naturally occurring mutations. We also present evidence for CRISPR/Cas9 being a highly precise tool, as we did not detect off-target mutations in Tomelo. Using our pipeline, mutations can be readily introduced into elite or locally adapted tomato varieties in less than a year with relatively minimal effort and investment.

PMID:
28352080
PMCID:
PMC5428673
DOI:
10.1038/s41598-017-00578-x
[Indexed for MEDLINE]
Free PMC Article

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