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J Genomics. 2017 Mar 9;5:32-35. doi: 10.7150/jgen.19407. eCollection 2017.

Complete genome sequence and functional study of the fibrinolytic enzyme-producing bacterium Leuconostoc holzapfelii 5H4, a silage probiotic.

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Division of Applied Life Science (BK21 Plus) and Institute of Agriculture & Life Science, Gyeongsang National University, Jinju 52828, Korea.
Central Area Crop Breeding Division, NICS, RDA, Suwon 16429, Korea.


To process silage, rye is usually removed before the heading stage but the rye biomass increased up to 30% after the heading stage. However, after the heading stage, lignification rapidly accelerated and it resulted in a poor NDF digestibility problem. This has led to a demand for a strong fibrinolytic enzyme-producing probiotic for rye silage. The Gram-positive Leuconostoc holzapfelii 5H4 was selected as a fibrinolytic enzyme-producing probiotic to overcome lignification of rye silage. The L. holzapfelii 5H4 has a relatively small circular chromosome (1,885,398 bp), but the strain has one cellulase, two xylanase, and five esterase in its genome sequence. All fibrinolytic enzyme genes were relatively highly expressed compared to housekeeping genes, and this was confirmed by qRT-PCR. In this study, we report the complete genome sequence of the bacterium so that fibrinolytic enzyme production and its fibrinolytic activity mechanism are better understood.


Complete genome sequencing; Lignification; NDF digestibility

Conflict of interest statement

Competing Interests: The authors have declared that no competing interest exists.

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