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J Exp Biol. 2017 Jun 1;220(Pt 11):2057-2065. doi: 10.1242/jeb.150318. Epub 2017 Mar 27.

Olfactory sensitivity of the marine flatfish Solea senegalensis to conspecific body fluids.

Author information

1
IRTA Sant Carles de la Ràpita Crta, De Poble Nou km. 5.5 (43540) Sant Carles de la Ràpita, Tarragona, Spain.
2
IRTA-Institut de Ciències del Mar, Consejo Superior de Investigaciones Científicas (CSIC), Barcelona 08003, Spain.
3
IFAPA Centro El Toruño, Junta de Andalucía, Camino Tiro Pichón s/n, 11500 El Puerto Santa María, Cádiz, Spain.
4
Centre of Marine Sciences (CCMAR), Universidade do Algarve, Campus de Gambelas, Faro 8005-139, Portugal phubbard@ualg.pt.

Abstract

Chemical communication is better understood in freshwater fish than marine fish. The Senegalese sole (Solea senegalensis) is a marine flatfish wherein one of the problems in aquaculture is the poor reproductive performance of hatchery-bred males. Is chemical communication involved in the reproduction of this species? Urine, intestinal fluid and mucus samples were taken from adult fish (wild-caught and hatchery-bred) over the spawning season (March-May), and assessed for olfactory potency using the electro-olfactogram (EOG). The effect of stimulation of the olfactory system with adult female urine on circulating luteinizing hormone (LH) levels was also tested in males. Intestinal fluid and urine were potent olfactory stimuli for both juvenile and adult conspecifics, evoking large-amplitude, concentration-dependent EOG responses, with thresholds of detection at approximately 1:106 However, the amplitude of the response to urine depended on the sex and state of maturity of both the donor and the receiver. Most olfactory activity could be extracted by C18 solid-phase cartridges. Urine from mature females evoked a slight, but significant, increase in circulating LH levels in mature males 30 min after exposure. Furthermore, the olfactory potency of urine differed between wild-caught and hatchery-bred fish; however, contrary to expectations, urine from wild-caught females was less potent than that from hatchery-bred females. Taken together, these results strongly suggest that faeces- and urine-released odorants are involved in reproduction in the Senegalese sole, and establish a basis for further investigation into pheromonal communication in marine teleosts.

KEYWORDS:

Faeces; Marine; Olfaction; Pheromone; Reproduction; Urine

PMID:
28348043
DOI:
10.1242/jeb.150318
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Conflict of interest statement

Competing interestsThe authors declare no competing or financial interests.

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