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J Pharm Biomed Anal. 2017 Jun 5;140:105-112. doi: 10.1016/j.jpba.2017.02.058. Epub 2017 Mar 10.

Human exposure to Bisphenol A and liver health status: Quantification of urinary and circulating levels by LC-MS/MS.

Author information

1
Department of Experimental Medicine, University of Campania "L. Vanvitelli", Via S. M. di Costantinopoli, 16, 80138 Naples, Italy.
2
Department of Clinical and Experimental Medicine, Division of Hepatogastroenterology, University of Campania "L. Vanvitelli", Via Pansini, 5, 80131 Naples, Italy.
3
Department of Experimental Medicine, University of Campania "L. Vanvitelli", Via S. M. di Costantinopoli, 16, 80138 Naples, Italy. Electronic address: nadia.diano@unicampania.it.

Abstract

A selective and highly sensitive analytical methodology for determination of Bisphenol A in human plasma was developed and validated. The method was based on selective liquid/solid extraction, combined with liquid chromatography-electrospray ionization tandem mass spectrometry in the multiple reaction monitoring mode and negative ionization. The linearity of the detector response was verified in human plasma over the concentration range 0.100-200ngmL-1. The detection limit was 0.03ngmL-1 and the quantification limit was 0.100ngmL-1. The analytical features of the proposed in-house validated method were satisfactory: precision was <10% and recoveries were around 84-104%. The matrix effect was studied and compensated using deuterated labeled standard. The applicability of the proposed method was demonstrated analyzing human plasma samples from individuals affected by non-alcoholic fatty liver disease. Bisphenol A was detected above the detection limit in all samples. The data show a persistence of unconjugated Bisphenol A levels in plasma and indicate a chronic Bisphenol A exposure of the target organ, suggesting an association between liver health status and Bisphenol A exposure. The results from our study are valuable for further investigation with large sample size and longitudinal study designs, necessary to confirm the observed association.

KEYWORDS:

Bisphenol A; Human plasma; Liquid chromatography–tandem mass spectrometry; Liver

PMID:
28346880
DOI:
10.1016/j.jpba.2017.02.058
[Indexed for MEDLINE]

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