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Nat Biotechnol. 2017 May;35(5):441-443. doi: 10.1038/nbt.3833. Epub 2017 Mar 27.

Targeted base editing in rice and tomato using a CRISPR-Cas9 cytidine deaminase fusion.

Author information

1
Graduate School of Science, Technology and Innovation, Kobe University, Kobe, Japan.
2
Faculty of Life and Environmental Sciences, University of Tsukuba, Gene Research Center, Tsukuba, Japan.
3
Laboratory of Genetics and Breeding Science, Faculty of Agriculture, Meijo University, Nagoya, Japan.
4
Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, Kobe, Japan.

Abstract

We applied a fusion of CRISPR-Cas9 and activation-induced cytidine deaminase (Target-AID) for point mutagenesis at genomic regions specified by single guide RNAs (sgRNAs) in two crop plants. In rice, we induced multiple herbicide-resistance point mutations by multiplexed editing using herbicide selection, while in tomato we generated marker-free plants with homozygous heritable DNA substitutions, demonstrating the feasibility of base editing for crop improvement.

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PMID:
28346401
DOI:
10.1038/nbt.3833
[Indexed for MEDLINE]

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