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J Gen Microbiol. 1987 Sep;133(9):2421-6.

Characterization of the product of the cloned fdhF gene of Escherichia coli.

Author information

1
Laboratoire de Microbiologie, Institut National des Sciences Appliquées, Villeurbanne, France.

Abstract

Random insertions of mini-MudII1734 lac gene fusion bacteriophage were constructed on plasmid pLW06, which carries the fdhF gene coding for benzyl-viologen-linked formate dehydrogenase. They allowed us to limit the size of the gene to a 3 kb fragment and to define its direction of transcription. The identification of the fdhF product as a 85 kDa protein was achieved after expression of derivative plasmids in a maxicell system.

PMID:
2834507
DOI:
10.1099/00221287-133-9-2421
[Indexed for MEDLINE]

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