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Environ Int. 2017 Jun;103:91-98. doi: 10.1016/j.envint.2017.03.012. Epub 2017 Mar 23.

Hydroxy-fipronil is a new urinary biomarker of exposure to fipronil.

Author information

1
Department of Entomology and Nematology, and UCD Comprehensive Cancer Center, University of California Davis, Davis, CA 95616, United States.
2
Faculty of Agriculture, Plant Protection Department, Zagazig University, Egypt.
3
Department of Pharmacology, University of California Davis, Davis, CA 95616, United States.
4
United States Environmental Protection Agency, National Exposure Research Laboratory, Research Triangle Park, NC, United States.
5
Department of Entomology and Nematology, and UCD Comprehensive Cancer Center, University of California Davis, Davis, CA 95616, United States. Electronic address: bdhammock@ucdavis.edu.

Abstract

Occupational medical surveillance is highly desirable in manufacturing facilities where exposure to chemicals is significant. The insecticide fipronil is generally considered safe for humans but with increasing use, exposure to fipronil is of concern. Identification of urinary metabolites of fipronil may allow development of affordable, cheap and rapid procedures for human exposure evaluation. In this study we developed a fast and easy approach for synthesis of hydroxy-fipronil, a potential urinary metabolite of fipronil. This standard was used to develop a sensitive analytical LC-MS/MS method with a limit of quantification (LOQ) of 0.4ng/mL. Fipronil sulfone, a known metabolite, and hydroxy-fipronil were quantified in urine samples from rats treated with a fipronil containing diet. Fipronil sulfone concentration centered around 20ng/mL, while the concentration of hydroxy-fipronil was dose-dependent ranging in 10-10,000ng/mL and thus being a more sensitive marker of fipronil exposure. A fipronil immunoassay with cross-reactivity to hydroxy-fipronil showed a good correlation in signal intensity with LC-MS data. It was also used to demonstrate the applicability of the method for sample screening in the evaluation of exposure levels.

KEYWORDS:

Antibody; GABA receptor bioassay; Immunoassay; Insecticide; LC-MS/MS; Metabolite; Screening; Sensor

PMID:
28343720
PMCID:
PMC5432128
DOI:
10.1016/j.envint.2017.03.012
[Indexed for MEDLINE]
Free PMC Article

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