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J Clin Virol. 2017 Aug;93:85-86. doi: 10.1016/j.jcv.2017.02.005. Epub 2017 Feb 15.

Diagnosing acute HIV infection: The performance of quantitative HIV-1 RNA testing (viral load) in the 2014 laboratory testing algorithm.

Author information

1
Division of HIV/AIDS Prevention, Centers for Disease Control and Prevention, Atlanta, GA, USA. Electronic address: prestopkimo@gmail.com.
2
San Francisco Department of Public Health, San Francisco, CA, USA.
3
New York City Department of Health & Mental Hygiene, New York City, NY, USA.
4
University of North Carolina at Chapel Hill, Chapel Hill, NC, USA(1).
5
Division of HIV/AIDS Prevention, Centers for Disease Control and Prevention, Atlanta, GA, USA; ICF International, Atlanta, GA, USA.
6
Division of HIV/AIDS Prevention, Centers for Disease Control and Prevention, Atlanta, GA, USA.
7
Division of HIV/AIDS Prevention, Centers for Disease Control and Prevention, Atlanta, GA, USA. Electronic address: pjpeters@cdc.gov.

Abstract

New recommendations for laboratory diagnosis of HIV infection in the United States were published in 2014. The updated testing algorithm includes a qualitative HIV-1 RNA assay to resolve discordant immunoassay results and to identify acute HIV-1 infection (AHI). The qualitative HIV-1 RNA assay is not widely available; therefore, we evaluated the performance of a more widely available quantitative HIV-1 RNA assay, viral load, for diagnosing AHI. We determined that quantitative viral loads consistently distinguished AHI from a false-positive immunoassay result. Among 100 study participants with AHI and a viral load result, the estimated geometric mean viral load was 1,377,793copies/mL.

KEYWORDS:

Acute HIV infection; Fourth-generation immunoassay; HIV diagnosis; HIV testing; Quantitative HIV-1 viral load

PMID:
28342746
DOI:
10.1016/j.jcv.2017.02.005
[Indexed for MEDLINE]

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