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Chemphyschem. 2017 Jul 5;18(13):1735-1741. doi: 10.1002/cphc.201700072. Epub 2017 May 3.

A DNA Logic Gate Automaton for Detection of Rabies and Other Lyssaviruses.

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Division of Experimental Therapeutics, Department of Medicine, Columbia University, 630 W 168thSt, New York, NY, 10032, USA.
Genecology Research Centre; Inflammation and Healing Research Cluster, School of Science and Engineering, University of the Sunshine Coast, Queensland, Australia.


Immediate activation of biosensors is not always desirable, particularly if activation is due to non-specific interactions. Here we demonstrate the use of deoxyribozyme-based logic gate networks arranged into visual displays to precisely control activation of biosensors, and demonstrate a prototype molecular automaton able to discriminate between seven different genotypes of Lyssaviruses, including Rabies virus. The device uses novel mixed-base logic gates to enable detection of the large diversity of Lyssavirus sequence populations, while an ANDNOT logic gate prevents non-specific activation across genotypes. The resultant device provides a user-friendly digital-like, but molecule-powered, dot-matrix text output for unequivocal results read-out that is highly relevant for point of care applications.


DNA logic gate; biosensors; molecular automaton; viral diagnostics; visual display

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