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Reprod Toxicol. 2017 Apr;69:254-264. doi: 10.1016/j.reprotox.2017.03.010. Epub 2017 Mar 21.

Alterations in prostate morphogenesis in male rat offspring after maternal exposure to Di-n-butyl-phthalate (DBP).

Author information

1
Department of Anatomy, Institute of Biosciences, Univi Estadual Paulista/UNESP, Botucatu, SP, Brazil. Electronic address: talitamello.tms@gmail.com.
2
Department of Morphology, Institute of Biosciences, Univi Estadual Paulista/UNESP, Botucatu, SP, Brazil.
3
Department of Anatomy, Institute of Biosciences, Univi Estadual Paulista/UNESP, Botucatu, SP, Brazil.

Abstract

Prostate morphogenesis is regulated by androgens hormones and modulated by morphogenetic proteins such as Bone Morphogenetic Proteins (BMPs). This study aims to investigate the effects on prostate development in male offspring and differentiation after gestational and lactational maternal exposure to Di-n-butyl-phthalate (DBP), an important environmental contamination. Pregnant Wistar rats received 100 or 500mg/kg of DBP (DBP100 and DBP500), by gavage, from gestation day 15 (GD15) until postnatal day 21 (PND21). The pups were euthanized on PND1 and PND21. Anogenital distance and testosterone levels decreased in animals from exposed mothers (DBP100 and 500) on PND1. A three-dimensional reconstruction model of the prostatic urethra showed reduction in the prostatic buds in the DBP500 group. AR expression and α-actin immunoreactivity decreased, and BMP-4 expression was lower on PND1 for DBP500. These results showed that DBP exposure, especially at a higher dose, delayed prostate morphogenesis by reducing the testosterone/AR axis and BMP-4 expression.

KEYWORDS:

Androgen receptor; Antiandrogenic; Di-n-butyl-phthalate (DBP) BMP-4; Prostate development; Testosterone; α-actin

PMID:
28341571
DOI:
10.1016/j.reprotox.2017.03.010
[Indexed for MEDLINE]

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