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Curr Genet. 1987;12(7):489-95.

Characterization of a chloroplast mutation in the psaA2 gene of Chlamydomonas reinhardtii.

Author information

1
Institut de Biologie Physico-Chimique, Paris, France.

Abstract

The synthesis of polypeptides related to the CPI chlorophyll-protein complex of photosystem I has been studied by pulse-labeling experiments in twenty chloroplast mutants of Chlamydomonas reinhardtii. Three mutations of the same locus (Girard-Bascou 1987) result in the absence of these CPI-related polypeptides. Among these mutations one, (FUD26) leads to the synthesis of a new polypeptide presumed to be a truncated CPI apoprotein. The molecular characterization of this mutation in the psaA2 gene has been achieved by DNA sequencing the 3' end of this gene. The FUD26 mutation is a 4 base pair deletion resulting in frameshift and premature termination of the protein.

PMID:
2834098
DOI:
10.1007/bf00419557
[Indexed for MEDLINE]

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