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Curr Genet. 1986;11(1):65-70.

Comparison of expression of the endo-beta-1,3-1,4-glucanase gene from Bacillus subtilis in Saccharomyces cerevisiae from the CYC1 and ADH1 promoters.

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1
Research Centre, Arthur Guiness Son & Co. Ltd., Dublin, Ireland.

Abstract

The endo-beta-1,3-1,4-glucanase gene from B. subtilis was placed under yeast promoter control in a number of different yeast expression vectors. The hybrid plasmids were transformed into S. cerevisiae where they directed the synthesis of varying amounts of active enzyme. The presence of B. subtilis DNA sequences 5' to the initiation codon for the B. subtilis beta-glucanase gene reduced expression of the gene in yeast. A 1,000-fold increase in the yield of beta-glucanase was obtained using the ADH1 promoter compared with the CYC1 promoter.

PMID:
2834081
DOI:
10.1007/bf00389427
[Indexed for MEDLINE]

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