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Cell. 2017 Mar 23;169(1):161-173.e12. doi: 10.1016/j.cell.2017.03.008.

Tridimensional Visualization and Analysis of Early Human Development.

Author information

1
Sorbonne Universités, UPMC Univ Paris 06, INSERM, CNRS, Institut de la Vision, 17 Rue Moreau, 75012 Paris, France.
2
UMR-S 1172, JPArc - Centre de Recherche Jean-Pierre AUBERT Neurosciences et Cancer, University of Lille, Lille 59000, France; INSERM, UMR-S 1172, Laboratory of Development and Plasticity of the Neuroendocrine Brain, Lille 59000, France.
3
FHU 1,000 Days for Health, School of Medicine, University of Lille, Lille 5900, France; Gynaecology Service, Hospital Jeanne de Flandres, CHU Lille, Lille 59000, France.
4
UMR-S 1172, JPArc - Centre de Recherche Jean-Pierre AUBERT Neurosciences et Cancer, University of Lille, Lille 59000, France; INSERM, UMR-S 1172, Laboratory of Development and Plasticity of the Neuroendocrine Brain, Lille 59000, France; FHU 1,000 Days for Health, School of Medicine, University of Lille, Lille 5900, France.
5
Sorbonne Universités, UPMC Univ Paris 06, INSERM, CNRS, Institut de la Vision, 17 Rue Moreau, 75012 Paris, France. Electronic address: alain.chedotal@inserm.fr.

Abstract

Generating a precise cellular and molecular cartography of the human embryo is essential to our understanding of the mechanisms of organogenesis in normal and pathological conditions. Here, we have combined whole-mount immunostaining, 3DISCO clearing, and light-sheet imaging to start building a 3D cellular map of the human development during the first trimester of gestation. We provide high-resolution 3D images of the developing peripheral nervous, muscular, vascular, cardiopulmonary, and urogenital systems. We found that the adult-like pattern of skin innervation is established before the end of the first trimester, showing important intra- and inter-individual variations in nerve branches. We also present evidence for a differential vascularization of the male and female genital tracts concomitant with sex determination. This work paves the way for a cellular and molecular reference atlas of human cells, which will be of paramount importance to understanding human development in health and disease. PAPERCLIP.

KEYWORDS:

3DISCO; database; embryo; fetus; human; iDISCO; light sheet microscopy; nervous system; tissue clearing; urogenital system

PMID:
28340341
DOI:
10.1016/j.cell.2017.03.008
[Indexed for MEDLINE]
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