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Monoclon Antib Immunodiagn Immunother. 2017 Apr;36(2):68-71. doi: 10.1089/mab.2016.0052. Epub 2017 Mar 24.

Development of RAP Tag, a Novel Tagging System for Protein Detection and Purification.

Author information

1
1 Department of Regional Innovation, Tohoku University Graduate School of Medicine , Sendai, Japan .
2
2 New Industry Creation Hatchery Center, Tohoku University , Sendai, Japan .

Abstract

Affinity tag systems, possessing high affinity and specificity, are useful for protein detection and purification. The most suitable tag for a particular purpose should be selected from many available affinity tag systems. In this study, we developed a novel affinity tag called the "RAP tag" system, which comprises a mouse antirat podoplanin monoclonal antibody (clone PMab-2) and the RAP tag (DMVNPGLEDRIE). This system is useful not only for protein detection in Western blotting, flow cytometry, and sandwich enzyme-linked immunosorbent assay, but also for protein purification.

KEYWORDS:

RAP tag; affinity tag; monoclonal antibody; podoplanin; protein purification

PMID:
28339303
PMCID:
PMC5404252
DOI:
10.1089/mab.2016.0052
[Indexed for MEDLINE]
Free PMC Article

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