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J Exp Bot. 2017 Mar 1;68(7):1697-1713. doi: 10.1093/jxb/erx038.

Metabolite profiling of barley flag leaves under drought and combined heat and drought stress reveals metabolic QTLs for metabolites associated with antioxidant defense.

Author information

1
Julius Kühn-Institute, Federal Research Centre for Cultivated Plants, Institute of Resistance Research and Stress Tolerance, Erwin-Baur-Str. 27, D-06484 Quedlinburg, Germany.
2
Max Planck Institute for Breeding Research, Carl-von-Linné-Weg 10, D-50829 Köln, Germany.
3
Friedrich-Alexander-Universität Erlangen-Nürnberg, Division of Biochemistry, Staudtstr. 5, D-91058 Erlangen, Germany.
4
Saatzucht Josef Breun GmbH & Co. KG, Amselweg 1, D-91074 Herzogenaurach, Germany.
5
SAATEN-UNION BIOTEC GmbH, Hovedisser Strasse 92, D-33818 Leopoldshöhe, Germany.
6
Cluster of Excellence on Plant Sciences, Heinrich-Heine-Universität Düsseldorf, Institute for Plant Genetics, Universitätsstrasse 1, D-40225 Düsseldorf, Germany.

Abstract

Barley (Hordeum vulgare L.) is among the most stress-tolerant crops; however, not much is known about the genetic and environmental control of metabolic adaptation of barley to abiotic stresses. We have subjected a genetically diverse set of 81 barley accessions, consisting of Mediterranean landrace genotypes and German elite breeding lines, to drought and combined heat and drought stress at anthesis. Our aim was to (i) investigate potential differences in morphological, physiological, and metabolic adaptation to the two stress scenarios between the Mediterranean and German barley genotypes and (ii) identify metabolic quantitative trait loci (mQTLs). To this end, we have genotyped the investigated barley lines with an Illumina iSelect 9K array and analyzed a set of 57 metabolites from the primary C and N as well as antioxidant metabolism in flag leaves under control and stress conditions. We found that drought-adapted genotypes attenuate leaf carbon metabolism much more strongly than elite lines during drought stress adaptation. Furthermore, we identified mQTLs for flag leaf γ-tocopherol, glutathione, and succinate content by association genetics that co-localize with genes encoding enzymes of the pathways producing these antioxidant metabolites. Our results provide the molecular basis for breeding barley cultivars with improved abiotic stress tolerance.

KEYWORDS:

Barley; GABA shunt; combined heat and drought stress; genome-wide association mapping; glutathione; mQTL; metabolite profiling; thousand grain weight; tocopherol.

PMID:
28338908
PMCID:
PMC5441916
DOI:
10.1093/jxb/erx038
[Indexed for MEDLINE]
Free PMC Article

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