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Chin J Integr Med. 2017 Mar 23. doi: 10.1007/s11655-017-2801-5. [Epub ahead of print]

Inhibition of proliferation of prostate cancer cell line DU-145 in vitro and in vivo using Salvia miltiorrhiza Bunge.

Author information

1
Department of Urology, The Catholic University of Korea, College of Medicine, Seoul, 06591, Republic of Korea.
2
Catholic Integrative Medicine Research Institute, College of Medicine, The Catholic University of Korea, Seoul, 06591, Republic of Korea.
3
Korea Bio-Medical Science Institute, Seoul, 06106, Republic of Korea.
4
Department of Urology, Second Hospital of Lanzhou University, Lanzhou, 730030, China.
5
Department of Urology, The Catholic University of Korea, College of Medicine, Seoul, 06591, Republic of Korea. ksw1227@catholic.ac.kr.
6
Catholic Integrative Medicine Research Institute, College of Medicine, The Catholic University of Korea, Seoul, 06591, Republic of Korea. ksw1227@catholic.ac.kr.

Abstract

OBJECTIVE:

To investigate the antiproliferative activity of Salvia miltiorrhiza Bunge. (SM) on the castration-resistant prostate cancer (CRPC) cell line DU-145, in vitro and in vivo.

METHODS:

Prostate cancer cell line (DU-145) and normal prostate cell line (RWPE-1) were treated with SM at different concentrations (3.125, 12.5, 25 and 50 μg/mL) to investigate the antiproliferative effects. DNA laddering analysis was performed to investigate the apoptosis of DU-145 cells. Molecular mechanism was investigated by Western blot analysis of p53, Bcl-2, prostate specific antigen (PSA), and androgen receptor (AR). Six-week-old male BALB/c nude mice were randomly divided into normal control group (n=101) and treated group (n=101) which administered 500 mg/kg SM for 2 weeks. Tumor volumes were measured.

RESULTS:

Treatment with SM resulted in a dose-dependent decrease in cell number of DU-145 cells in comparison with RWPE-1. DNA laddering analysis indicated the apoptosis of DU-145 cells. Treatment with SM increased the expression of p53 and reduced the expression of Bcl-2 proteins. The levels of PSA were considerably reduced in SM-treated group compared to the controls, and a decrease in AR expression was observed when cells were treated with SM in the same pattern as a reduction in PSA. In the tumour xenograft study, SM given once a day for 2 weeks significantly inhibited tumour growth.

CONCLUSION:

SM might contribute to the anticancer actions such as induction of apoptosis and inhibition of proliferation of prostate cancer cells.

KEYWORDS:

Salvia miltiorrhiza Bunge.; apoptosis; prostate cancer

PMID:
28337641
DOI:
10.1007/s11655-017-2801-5

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