Hepatoprotective Effects of Antrodia cinnamomea: The Modulation of Oxidative Stress Signaling in a Mouse Model of Alcohol-Induced Acute Liver Injury

Oxid Med Cell Longev. 2017:2017:7841823. doi: 10.1155/2017/7841823. Epub 2017 Feb 27.

Abstract

In the present study, the components of A. cinnamomea (AC) mycelia were systematically analyzed. Subsequently, its hepatoprotective effects and the underlying mechanisms were explored using a mouse model of acute alcohol-induced liver injury. AC contained 25 types of fatty acid, 16 types of amino acid, 3 types of nucleotide, and 8 types of mineral. The hepatoprotective effects were observed after 2 weeks of AC treatment at doses of 75 mg/kg, 225 mg/kg, and 675 mg/kg in the mouse model. These effects were indicated by the changes in the levels of aspartate aminotransferase, alanine aminotransferase, several oxidation-related factors, and inflammatory cytokines in serum and/or liver samples. AC reduced the incidence rate of necrosis, inflammatory infiltration, fatty droplets formation, and cell apoptosis in liver detecting via histological and TUNEL assay. In addition, AC reduced the expression of cleaved caspase-3, -8, and -9 and the levels of phosphor-protein kinase B (Akt) and phosphor-nuclear factor-κB (NF-κB) in the liver samples. Collectively, AC-mediated hepatoprotective effects in a mouse model of acute alcohol-induced liver injury are the result of reduction in oxidative stress. This may be associated with Akt/NF-κB signaling. These results provide valuable evidence to support the use of A. cinnamomea as a functional food and/or medicine.

MeSH terms

  • Alanine Transaminase / blood
  • Alcohols / toxicity
  • Animals
  • Antioxidants / metabolism
  • Antrodia / chemistry*
  • Antrodia / metabolism
  • Aspartate Aminotransferases / blood
  • Caspase 3 / metabolism
  • Caspase 8 / metabolism
  • Caspase 9 / metabolism
  • Chemical and Drug Induced Liver Injury / metabolism
  • Chemical and Drug Induced Liver Injury / pathology
  • Cytokines / blood
  • Cytokines / metabolism
  • Disease Models, Animal
  • Liver / metabolism
  • Liver / pathology
  • Mice
  • NF-kappa B / metabolism
  • Oxidative Stress / drug effects*
  • Plant Extracts / chemistry
  • Plant Extracts / pharmacology*
  • Protective Agents / chemistry
  • Protective Agents / pharmacology*
  • Proto-Oncogene Proteins c-akt / metabolism
  • Reactive Oxygen Species / metabolism
  • Signal Transduction / drug effects*

Substances

  • Alcohols
  • Antioxidants
  • Cytokines
  • NF-kappa B
  • Plant Extracts
  • Protective Agents
  • Reactive Oxygen Species
  • Aspartate Aminotransferases
  • Alanine Transaminase
  • Proto-Oncogene Proteins c-akt
  • Caspase 3
  • Caspase 8
  • Caspase 9