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Science. 2017 Mar 24;355(6331). pii: eaam5488. doi: 10.1126/science.aam5488.

Self-assembly of genetically encoded DNA-protein hybrid nanoscale shapes.

Author information

1
Physics Department and Institute for Advanced Study, Technische Universität München, Garching bei München, Germany.
2
Physics Department and Institute for Advanced Study, Technische Universität München, Garching bei München, Germany. dietz@tum.de.

Abstract

We describe an approach to bottom-up fabrication that allows integration of the functional diversity of proteins into designed three-dimensional structural frameworks. A set of custom staple proteins based on transcription activator-like effector proteins folds a double-stranded DNA template into a user-defined shape. Each staple protein is designed to recognize and closely link two distinct double-helical DNA sequences at separate positions on the template. We present design rules for constructing megadalton-scale DNA-protein hybrid shapes; introduce various structural motifs, such as custom curvature, corners, and vertices; and describe principles for creating multilayer DNA-protein objects with enhanced rigidity. We demonstrate self-assembly of our hybrid nanostructures in one-pot mixtures that include the genetic information for the designed proteins, the template DNA, RNA polymerase, ribosomes, and cofactors for transcription and translation.

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PMID:
28336611
DOI:
10.1126/science.aam5488
[Indexed for MEDLINE]

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