Highly Modular Bioluminescent Sensors for Small Molecules and Proteins

Methods Enzymol. 2017:589:365-382. doi: 10.1016/bs.mie.2017.01.005. Epub 2017 Feb 28.

Abstract

Obtaining patient-specific information through the quantification of small molecules and proteins in bodily fluids is essential for personalized therapies. Point-of-care (POC) diagnostic devices hold the promise of delivering such benefit to a wide range of patients. However, there is a lack of enabling technology, as the majority of newly developed POC devices focus on the same underlying core technologies. Here we provide an overview of a new technology based on highly modular bioluminescent sensors that enables the quantification of small molecules and proteins at the POC with low-cost devices.

Keywords: Bioluminescence; Biosensors; Point-of-care device; Protein engineering.

Publication types

  • Review

MeSH terms

  • Animals
  • Biosensing Techniques / instrumentation
  • Biosensing Techniques / methods*
  • Drug Monitoring / instrumentation
  • Drug Monitoring / methods*
  • Humans
  • Luminescent Measurements / instrumentation
  • Luminescent Measurements / methods*
  • Models, Molecular
  • Pharmaceutical Preparations / analysis*
  • Point-of-Care Systems*
  • Proteins / analysis*

Substances

  • Pharmaceutical Preparations
  • Proteins